Improved β - Glucuroni - dase Reporter for Mammalian Gene Expression Analysis

نویسندگان

  • Christopher K. Raymond
  • Virginia R. Mugford
  • Shannon L. Sexson
چکیده

1.Agatep, R., R.D. Kirkpatrick, D.L. Parchaliuk, R.A. Woods and R.D. Gietz. 1998. Transformation of Saccharomyces cerevisiae by the lithium acetate/single-stranded carrier DNA/polyethylene glycol (LiAc/ss-DNA/ PEG) protocol. Technical Tips Online (http://tto.trends.com). 2.Boulton, S. and S. Jackson. 1996. Identification of a Saccharomyces cerevisiae Ku80 homologue: roles in DNA double strand break joining and in telomeric maintenance. Nucleic Acids Res. 24:4639-4648. 3.Christianson, T.W., R.S. Sikorski, M. Dante, J.H. Shero and P. Hieter. 1992. Multifunctional yeast high-copy number shuttle vectors. Gene 110:119-122. 4.Gietz, R., R. Schiestl, A. Willems and R. Woods. 1995. Studies on the transformation of intact yeast cells by the LiAc/SSDNA/PEG procedure. Yeast 11:355-360. 5.Meilhoc, E., J.-M. Masson and J. Teisse. 1990. High efficiency transformation of intact yeast cells by electric field pulses. BioTechnology 8:223-227. 6.Mezard, C. and A. Nicolas. 1994. Homologous, homeologous, and illegitimate repair of double-strand breaks during transformation of a wild-type strain and a rad52 mutant strain of Saccharomyces cerevisiae. Mol. Cell Biol. 14:1278-1292. 7.Raymond, C., T. Pownder and S. Sexson. 1998. A flexible and general method for plasmid construction using homologous recombination. BioTechniques 26:134-141. 8.Rothman, J., I. Howald and T. Stevens. 1989. Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J. 8:2057-2065. 9.Schiestl, R. and R. Gietz. 1989. High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier. Curr. Genet. 16:339-346. 10.Sikorski, R. and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19-27.

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تاریخ انتشار 2000